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Image Search Results
Journal: Metabolites
Article Title: Comparative Analysis of Biological Sphingolipids with Glycerophospholipids and Diacylglycerol by LC-MS/MS
doi: 10.3390/metabo4010098
Figure Lengend Snippet: Flow chart illustrating sphingolipid, glycerophospholipid (GPL) and diacylglycerol (DAG) sample preparation and analysis. Butanol and ethyl acetate/hexane (1:1) were used for the first and second extractions, respectively. To reduce the ion suppression effects associated with large amounts of phosphatidylcholine (PtdCho) and phosphatidylethanolamine (PtdEtn) in the sample, a DEAE fractionation step was introduced for the preparation of acidic lipids. Depending on the analyte assayed, four different MS methods (I–IV) were used for higher sensitivity. Further details are available in the Materials and Methods Section. SRM, selected reaction monitoring; Sph, sphingosine; Cer, ceramide; HexCer, monohexosylceramide; SM, sphingomyelin; PtdSer, phosphatidylserine; S1P, sphingosine-1-phosphate; Cer1P, ceramide-1-phosphate; PtdOH, phosphatidic acid; PtdGro, phosphatidylglycerol; PtdIns, phosphatidylinositol; GM3, monosialoganglioside; STD, standard solution; FA, formic acid.
Article Snippet: The
Techniques: Sample Prep, Fractionation